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sensory neuron maintenance medium  (Axol Bioscience)


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    Structured Review

    Axol Bioscience sensory neuron maintenance medium
    Sensory Neuron Maintenance Medium, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sensory+neuron+maintenance+medium/Sensory+Neuron+Maintenance+Medium/pm42541910-81-12-18
    Average 94 stars, based on 5 article reviews
    sensory neuron maintenance medium - by Bioz Stars, 2026-10
    94/100 stars

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    other:

    Article Title: Global analyses of <scp>mRNA</scp> expression in human sensory neurons reveal <scp>eIF5A</scp> as a conserved target for inflammatory pain
    Article Snippet: The media consisted of sensory neuron maintenance medium (Axol Bioscience, Little Chesterford, Cambridge, UK) supplemented with astrocyte supplement (BrainXell, Inc., Madison, WI, USA) and the following growth factors: NGF (25 ng/ml), NT- 3 (10 ng/ ml), BDNF (10 ng/ml), and GDNF (25 ng/ml).

    Article Title: A multi-well axon array device for evaluation of stimulus responses on human sensory neural spheroid-derived axon fascicles.
    Article Snippet: On day 1 of the culture, the NPM medium was replaced with sensory neuron maintenance medium (SNMM, AX0060, Axol Bioscience, UK) containing growth factors.

    Article Title: Global analyses of mRNA expression in human sensory neurons reveals eIF5A as a conserved target for inflammatory pain
    Article Snippet: The media consisted of sensory neuron maintenance medium (Axol Bioscience, Little Chesterford, Cambridge, UK) supplemented with astrocyte supplement (BrainXell, Inc., Madison, WI, USA) and the following growth factors: NGF (25 ng/mL), NT-3 (10 ng/mL), BDNF (10 ng/mL), and GDNF (25 ng/mL).

    Recombinant:

    Article Title: Improved Tactile Receptivity and Skin Beauty Benefits Through Topical Treatment with a Hyacinthus orientalis Bulb Extract Shown to Activate Oxytocin Receptor Signaling
    Article Snippet: On days 1 and 3, the medium was completely replaced with enhanced sensory neuron maintenance medium supplemented with 0.25% 1:200 Surebond-XF; on day 3, 75% of the medium was replaced with enhanced sensory neuron maintenance medium supplemented with 2.5 μg/mL mitomycin C (Sigma M4287, St Louis, MO, USA); on day 6, 75% of the medium was replaced with enhanced sensory neuron maintenance medium supplemented with 0.25% 1:200 Surebond-XF; and on days 9, 13, 16, 20, and 23, 50% of the medium was replaced with enhanced sensory neuron maintenance medium. .. Enhanced sensory neuron maintenance medium consists of sensory neuron maintenance medium (Axol Bioscience ax0060) + 1% sensory maturation maximizer (Axol Bioscience ax0058) + 25 ng/mL Recombinant human Glial-Derived Neurotrophic Factor (Axol Bioscience ax139855) + 25 ng/mL Recombinant human Nerve Growth Factor (Axol Bioscience ax139789) + 10 ng/mL Recombinant human Brain-Derived Neurotrophic Factor (Axol Bioscience ax139800) + 10 ng/mL Recombinant human Neurotrophin-3 (Axol Bioscience ax139811). .. On day 20, keratinocytes (PromoCell C12005, Heidelberg, Germany) were seeded at 25,000 cells/channel.



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    Axol Bioscience motor neuron maintenance medium
    Number of iPSC <t>motor</t> <t>neuron</t> spheroids loaded in tissue engineered skeletal muscle determines functionality of neuromuscular tissues. ( a ) Schematic detailing method of iPSC motor neuron loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron <t>maintenance</t> medium. ( b ) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and ( c ) concentration dependent spontaneous twitch profiles compared to skeletal muscle only tissues. ( d ) Quantification of functional tetanic and ( e ) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Beginning of representative tetanus and twitch contractions represents initiation of electrical field stimulation, time elapsed before this point is un-stimulated baseline recording. Individual functional data points indicative of n = 3 contraction profiles, totalling minimum of n = 9 and maximum of n = 15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
    Motor Neuron Maintenance Medium, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Number of iPSC motor neuron spheroids loaded in tissue engineered skeletal muscle determines functionality of neuromuscular tissues. ( a ) Schematic detailing method of iPSC motor neuron loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth medium, DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. ( b ) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and ( c ) concentration dependent spontaneous twitch profiles compared to skeletal muscle only tissues. ( d ) Quantification of functional tetanic and ( e ) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Beginning of representative tetanus and twitch contractions represents initiation of electrical field stimulation, time elapsed before this point is un-stimulated baseline recording. Individual functional data points indicative of n = 3 contraction profiles, totalling minimum of n = 9 and maximum of n = 15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.

    Journal: Scientific Reports

    Article Title: Bioengineered model of the human motor unit with physiologically functional neuromuscular junctions

    doi: 10.1038/s41598-021-91203-5

    Figure Lengend Snippet: Number of iPSC motor neuron spheroids loaded in tissue engineered skeletal muscle determines functionality of neuromuscular tissues. ( a ) Schematic detailing method of iPSC motor neuron loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth medium, DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. ( b ) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and ( c ) concentration dependent spontaneous twitch profiles compared to skeletal muscle only tissues. ( d ) Quantification of functional tetanic and ( e ) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Beginning of representative tetanus and twitch contractions represents initiation of electrical field stimulation, time elapsed before this point is un-stimulated baseline recording. Individual functional data points indicative of n = 3 contraction profiles, totalling minimum of n = 9 and maximum of n = 15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.

    Article Snippet: At cellular confluence, motor neuron progenitors were dissociated using TrypLE select (1x, Gibco, Fisher), centrifuged at 200 G for 5 min and seeded in 96-well U bottom spheroid microplates (MoBiTec, MS-9096UZ) at 1 × 10 4 cells/well or monolayer at 1 × 10 5 cells/cm 2 in motor neuron maintenance medium (MM, Axol) supplemented with 0.5 µM RA, 5 ng/mL brain derived neurotrophic factor (BDNF, PeproTech) and 10 ng/mL ciliary neurotrophic factor (CNTF, Axol).

    Techniques: Cell Culture, Derivative Assay, Concentration Assay, Functional Assay, Standard Deviation